Application of RAPD-PCR in Taxonomy of Certain Freshwater Bivalves of Genus Caelatura
نویسندگان
چکیده
Adult specimens of the two common freshwater bivalves, Caelatura (Caelatura) companyoi and Caelatura (Caelatura) prasidens belonging to family Unionidae were collected from the River Nile at Giza governorate in Egypt. These two species were described by some authors as one species and others separated them as two different species. In the present study, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers were used to determine the genetic distance (D) between these two unionid species. 5 out of 13 random primers generated good DNA patterns that ranged from 1 to 3 bands (200-1000 bp). By application of the genetic distance equation, the result was above 0.5. Based on this result, the high value of D (0.64) proved that the present two specimens must be actually separated as 2 different species.
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